Summary information

PDB id
7xhd
Class
DNA
Method
X-ray (1.66 Å)
Summary
Crystal structure of a dimeric interlocked parallel g-quadruplex
Reference
Ngo KH, Liew CW, Lattmann S, Winnerdy FR, Phan AT (2022): "Crystal structures of an HIV-1 integrase aptamer: Formation of a water-mediated A•G•G•G•G pentad in an interlocked G-quadruplex." Biochem.Biophys.Res.Commun., 613, 153-158. doi: 10.1016/j.bbrc.2022.04.020.
Abstract
93del is a 16-nucleotide G-quadruplex-forming aptamer which can inhibit the activity of the HIV-1 integrase enzyme at nanomolar concentration. Previous structural analyses of 93del using NMR spectroscopy have shown that the aptamer forms an interlocked G-quadruplex structure in K+ solution. Due to its exceptional stability and unique topology, 93del has been used in many different studies involving DNA G-quadruplexes, such as DNA aptamer and multimer design, as well as DNA fluorescence research. To gain further insights on the structure of this unique aptamer, we have determined several high-resolution crystal structures of 93del and its variants. While confirming the overall dimeric interlocked G-quadruplex folding topology previously determined by NMR, our results reveal important detailed structural information, particularly the formation of a water-mediated A•G•G•G•G pentad. These insights allow us to better understand the formation of various structural elements in G-quadruplexes and should be useful for designing and manipulating G-quadruplex scaffolds with desired properties.
G4 notes
6 G-tetrads, 1 G4 helix, 2 G4 stems, 1 G4 coaxial stack, 2(-P-P-P), parallel(4+0), UUUU, coaxial interfaces: 5'/5'-SEPARATED

Base-block schematics in six views

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List of 6 G-tetrads

 1 glyco-bond=s--- sugar=---- groove=w--n planarity=0.103 type=planar nts=4 GGGG A.DG1,B.DG6,B.DG2,B.DG13
 2 glyco-bond=--s- sugar=---- groove=-wn- planarity=0.098 type=planar nts=4 GGGG A.DG2,A.DG6,B.DG1,A.DG13
 3 glyco-bond=---- sugar=--3- groove=---- planarity=0.093 type=planar nts=4 GGGG A.DG3,A.DG7,A.DG10,A.DG14
 4 glyco-bond=---- sugar=---- groove=---- planarity=0.196 type=other  nts=4 GGGG A.DG4,A.DG8,A.DG11,A.DG15
 5 glyco-bond=---- sugar=--3- groove=---- planarity=0.181 type=other  nts=4 GGGG B.DG3,B.DG7,B.DG10,B.DG14
 6 glyco-bond=---- sugar=---- groove=---- planarity=0.256 type=bowl   nts=4 GGGG B.DG4,B.DG8,B.DG11,B.DG15

List of 1 G4-helix

In DSSR, a G4-helix is defined by stacking interactions of G-tetrads, regardless of backbone connectivity, and may contain more than one G4-stem.

Helix#1, 6 G-tetrad layers, inter-molecular, with 2 stems

 1  glyco-bond=---- sugar=---- groove=---- WC-->Major nts=4 GGGG A.DG4,A.DG8,A.DG11,A.DG15
 2  glyco-bond=---- sugar=--3- groove=---- WC-->Major nts=4 GGGG A.DG3,A.DG7,A.DG10,A.DG14
 3  glyco-bond=--s- sugar=---- groove=-wn- WC-->Major nts=4 GGGG A.DG2,A.DG6,B.DG1,A.DG13
 4  glyco-bond=s--- sugar=---- groove=w--n Major-->WC nts=4 GGGG A.DG1,B.DG6,B.DG2,B.DG13
 5  glyco-bond=---- sugar=3--- groove=---- Major-->WC nts=4 GGGG B.DG10,B.DG7,B.DG3,B.DG14
 6  glyco-bond=---- sugar=---- groove=---- Major-->WC nts=4 GGGG B.DG11,B.DG8,B.DG4,B.DG15
  step#1  mp(<<,backward) area=10.64 rise=3.41 twist=29.5
  step#2  mp(<<,backward) area=9.25  rise=3.37 twist=32.8
  step#3  mm(<>,outward)  area=9.46  rise=3.47 twist=24.3
  step#4  pm(>>,forward)  area=10.78 rise=3.40 twist=29.2
  step#5  pm(>>,forward)  area=8.98  rise=3.39 twist=31.4
  strand#1 DNA glyco-bond=---s-- sugar=----3- nts=6 GGGGGG A.DG4,A.DG3,A.DG2,A.DG1,B.DG10,B.DG11
  strand#2 DNA glyco-bond=------ sugar=------ nts=6 GGGGGG A.DG8,A.DG7,A.DG6,B.DG6,B.DG7,B.DG8
  strand#3 DNA glyco-bond=--s--- sugar=-3---- nts=6 GGGGGG A.DG11,A.DG10,B.DG1,B.DG2,B.DG3,B.DG4
  strand#4 DNA glyco-bond=------ sugar=------ nts=6 GGGGGG A.DG15,A.DG14,A.DG13,B.DG13,B.DG14,B.DG15

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5 stacking diagrams
 1  glyco-bond=---- sugar=---- groove=---- WC-->Major nts=4 GGGG A.DG4,A.DG8,A.DG11,A.DG15
2 glyco-bond=---- sugar=--3- groove=---- WC-->Major nts=4 GGGG A.DG3,A.DG7,A.DG10,A.DG14
step#1 mp(<<,backward) area=10.64 rise=3.41 twist=29.5

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 2  glyco-bond=---- sugar=--3- groove=---- WC-->Major nts=4 GGGG A.DG3,A.DG7,A.DG10,A.DG14
3 glyco-bond=--s- sugar=---- groove=-wn- WC-->Major nts=4 GGGG A.DG2,A.DG6,B.DG1,A.DG13
step#2 mp(<<,backward) area=9.25 rise=3.37 twist=32.8

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 3  glyco-bond=--s- sugar=---- groove=-wn- WC-->Major nts=4 GGGG A.DG2,A.DG6,B.DG1,A.DG13
4 glyco-bond=s--- sugar=---- groove=w--n Major-->WC nts=4 GGGG A.DG1,B.DG6,B.DG2,B.DG13
step#3 mm(<>,outward) area=9.46 rise=3.47 twist=24.3

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 4  glyco-bond=s--- sugar=---- groove=w--n Major-->WC nts=4 GGGG A.DG1,B.DG6,B.DG2,B.DG13
5 glyco-bond=---- sugar=3--- groove=---- Major-->WC nts=4 GGGG B.DG10,B.DG7,B.DG3,B.DG14
step#4 pm(>>,forward) area=10.78 rise=3.40 twist=29.2

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 5  glyco-bond=---- sugar=3--- groove=---- Major-->WC nts=4 GGGG B.DG10,B.DG7,B.DG3,B.DG14
6 glyco-bond=---- sugar=---- groove=---- Major-->WC nts=4 GGGG B.DG11,B.DG8,B.DG4,B.DG15
step#5 pm(>>,forward) area=8.98 rise=3.39 twist=31.4

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List of 2 G4-stems

In DSSR, a G4-stem is defined as a G4-helix with backbone connectivity. Bulges are also allowed along each of the four strands.

Stem#1, 2 G-tetrad layers, 3 loops, INTRA-molecular, UUUU, parallel, 2(-P-P-P), parallel(4+0)

 1  glyco-bond=---- sugar=--3- groove=---- WC-->Major nts=4 GGGG A.DG3,A.DG7,A.DG10,A.DG14
 2  glyco-bond=---- sugar=---- groove=---- WC-->Major nts=4 GGGG A.DG4,A.DG8,A.DG11,A.DG15
  step#1  pm(>>,forward)  area=10.64 rise=3.41 twist=29.5
  strand#1  U DNA glyco-bond=-- sugar=-- nts=2 GG A.DG3,A.DG4
  strand#2  U DNA glyco-bond=-- sugar=-- nts=2 GG A.DG7,A.DG8
  strand#3  U DNA glyco-bond=-- sugar=3- nts=2 GG A.DG10,A.DG11
  strand#4  U DNA glyco-bond=-- sugar=-- nts=2 GG A.DG14,A.DG15
  loop#1 type=propeller strands=[#1,#2] nts=2 TG A.DT5,A.DG6
  loop#2 type=propeller strands=[#2,#3] nts=1 T A.DT9
  loop#3 type=propeller strands=[#3,#4] nts=2 TG A.DT12,A.DG13

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Stem#2, 2 G-tetrad layers, 3 loops, INTRA-molecular, UUUU, parallel, 2(-P-P-P), parallel(4+0)

 1  glyco-bond=---- sugar=--3- groove=---- WC-->Major nts=4 GGGG B.DG3,B.DG7,B.DG10,B.DG14
 2  glyco-bond=---- sugar=---- groove=---- WC-->Major nts=4 GGGG B.DG4,B.DG8,B.DG11,B.DG15
  step#1  pm(>>,forward)  area=8.98  rise=3.39 twist=31.4
  strand#1  U DNA glyco-bond=-- sugar=-- nts=2 GG B.DG3,B.DG4
  strand#2  U DNA glyco-bond=-- sugar=-- nts=2 GG B.DG7,B.DG8
  strand#3  U DNA glyco-bond=-- sugar=3- nts=2 GG B.DG10,B.DG11
  strand#4  U DNA glyco-bond=-- sugar=-- nts=2 GG B.DG14,B.DG15
  loop#1 type=propeller strands=[#1,#2] nts=2 TG B.DT5,B.DG6
  loop#2 type=propeller strands=[#2,#3] nts=1 T B.DT9
  loop#3 type=propeller strands=[#3,#4] nts=2 TG B.DT12,B.DG13

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List of 1 G4 coaxial stack

 1 G4 helix#1 contains 2 G4 stems: [#1,#2]  [5'/5'-SEPARATED]