Summary information [schematics · tetrads · helices · stems · costacks · homepage]

PDB-id
1a8w
Class
DNA
Method
NMR
Summary
A k+ cation-induced conformational switch within a loop spanning segment of a DNA quadruplex containing g-g-g-c repeats, NMR, 8 structures
Reference
Bouaziz, S., Kettani, A., Patel, D.J.: (1998) "A K cation-induced conformational switch within a loop spanning segment of a DNA quadruplex containing G-G-G-C repeats." J.Mol.Biol., 282, 637-652.
Abstract
We have identified a unique structural transition (in slow exchange on the NMR time scale) in the tertiary fold of the d(G-G-G-C-T4-G-G-G-C) quadruplex on proceeding from Na+ to K+ as counterion in aqueous solution. Both monovalent cation-dependent conformations exhibit certain common structural features, which include head-to-tail dimerization of two symmetry-related stem-hairpin loops, adjacent strands which are antiparallel to each other and adjacent stacked G(syn).G(anti). G(syn).G(anti) tetrads in the central core of the quadruplexes. The Na and K cation stabilized structures of the d(G-G-G-C-T4-G-G-G-C) quadruplexes differ in the conformations of the T-T-T-T loops, the relative alignment of G.C base-pairs positioned opposite each other through their major groove edges and potentially in the number of monovalent cation binding sites. We have identified potential K cation binding cavities within the symmetry-related T-T-T-G segments, suggesting the potential for two additional monovalent cation binding sites in the K cation-stabilized quadruplex relative to its Na cation-stabilized counterpart. Modeling studies suggest that the major groove edges of guanine residues in Watson-Crick G.C base-pairs could potentially be bridged by coordinated K cations in the d(G-G-G-C-T4-G-G-G-C) quadruplex in KCl solution in contrast to formation of G.C.G.C tetrads for the corresponding quadruplex in NaCl solution. Our results defining the molecular basis of a Na to K cation-dependent conformational switch in the loop spanning segment of the d(G-G-G-C-T4-G-G-G-C) quadruplex may have relevance to recent observations that specific K cation coordinated loop conformations within quadruplexes exhibit inhibitory activity against HIV integrase.
G4 notes
2 G-tetrads, 1 G4 helix, 1 G4 stem · (2+2), UDUD

Base-block schematics in six views [summary · tetrads · helices · stems · costacks · homepage]

PyMOL session file PDB file View in 3Dmol.js

List of 2 G-tetrads [summary · schematics · helices · stems · costacks · homepage]

 1 glyco-bond=s-s- groove=wnwn planarity=0.383 type=bowl-2 nts=4 GGGG A.DG2,A.DG11,B.DG10,B.DG3
 2 glyco-bond=-s-s groove=wnwn planarity=0.382 type=bowl-2 nts=4 GGGG A.DG3,A.DG10,B.DG11,B.DG2

List of 1 G4-helix [summary · schematics · tetrads · stems · costacks · homepage]

In DSSR, a G4-helix is defined by stacking interactions of G-tetrads, regardless of backbone connectivity, and may contain more than one G4-stem.

Helix#1, 2 G-tetrad layers, inter-molecular, with 1 stem

 1  glyco-bond=s-s- groove=wnwn Major-->WC nts=4 GGGG A.DG2,A.DG11,B.DG10,B.DG3
 2  glyco-bond=-s-s groove=wnwn WC-->Major nts=4 GGGG A.DG3,A.DG10,B.DG11,B.DG2
  step#1  mm(<>,outward)  area=14.60 rise=3.72 twist=22.1
  strand#1 DNA glyco-bond=s- nts=2 GG A.DG2,A.DG3
  strand#2 DNA glyco-bond=-s nts=2 GG A.DG11,A.DG10
  strand#3 DNA glyco-bond=s- nts=2 GG B.DG10,B.DG11
  strand#4 DNA glyco-bond=-s nts=2 GG B.DG3,B.DG2

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1 stacking diagram
 1  glyco-bond=s-s- groove=wnwn Major-->WC nts=4 GGGG A.DG2,A.DG11,B.DG10,B.DG3
2 glyco-bond=-s-s groove=wnwn WC-->Major nts=4 GGGG A.DG3,A.DG10,B.DG11,B.DG2
step#1 mm(<>,outward) area=14.60 rise=3.72 twist=22.1

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List of 1 G4-stem [summary · schematics · tetrads · helices · costacks · homepage]

In DSSR, a G4-stem is defined as a G4-helix with backbone connectivity. Bulges are also allowed along each of the four strands.

Stem#1, 2 G-tetrad layers, 2 loops, inter-molecular, UDUD, anti-parallel, (2+2)

 1  glyco-bond=s-s- groove=wnwn Major-->WC nts=4 GGGG A.DG2,A.DG11,B.DG10,B.DG3
 2  glyco-bond=-s-s groove=wnwn WC-->Major nts=4 GGGG A.DG3,A.DG10,B.DG11,B.DG2
  step#1  mm(<>,outward)  area=14.60 rise=3.72 twist=22.1
  strand#1  U DNA glyco-bond=s- nts=2 GG A.DG2,A.DG3
  strand#2  D DNA glyco-bond=-s nts=2 GG A.DG11,A.DG10
  strand#3  U DNA glyco-bond=s- nts=2 GG B.DG10,B.DG11
  strand#4  D DNA glyco-bond=-s nts=2 GG B.DG3,B.DG2
  loop#1 type=lateral   strands=[#1,#2] nts=6 CTTTTG A.DC4,A.DT5,A.DT6,A.DT7,A.DT8,A.DG9
  loop#2 type=lateral   strands=[#4,#3] nts=6 CTTTTG B.DC4,B.DT5,B.DT6,B.DT7,B.DT8,B.DG9

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List of 0 G4 coaxial stacks [summary · schematics · tetrads · helices · stems · homepage]

List of 0 non-stem G4-loops (including the two closing Gs)