DSSR-derived G-quadruplex features in PDB entry 1f3s
Poster "DSSR-Enabled Automatic Identification and Annotation of G-quadruplexes in the PDB" presented at the RNA2020 online meeting
Citation: before a paper dedicated to the DSSR-G4 module comes out, please cite the 2015 DSSR paper published in Nucleic Acids Research.
- Solution structure of DNA sequence gggttcagg forms gggg tetrade and g(c-a) triad
- Kettani, A., Basu, G., Gorin, A., Majumdar, A., Skripkin, E., Patel, D.J.: (2000) "A two-stranded template-based approach to G.(C-A) triad formation: designing novel structural elements into an existing DNA framework." J.Mol.Biol., 301, 129-146.
- We have designed a DNA sequence, d(G-G-G-T-T-C-A-G-G), which dimerizes to form a 2-fold symmetric G-quadruplex in which G(syn). G(anti).G(syn).G(anti) tetrads are sandwiched between all trans G. (C-A) triads. The NMR-based solution structural analysis was greatly aided by monitoring hydrogen bond alignments across N-H...N and N-H...O==C hydrogen bonds within the triad and tetrad, in a uniformly ((13)C,(15)N)-labeled sample of the d(G-G-G-T-T-C-A-G-G) sequence. The solution structure establishes that the guanine base-pairs with the cytosine through Watson-Crick G.C pair formation and with adenine through sheared G.A mismatch formation within the G.(C-A) triad. A model of triad DNA was constructed that contains the experimentally determined G.(C-A) triad alignment as the repeating stacked unit.
- G4 notes
- 2 G-tetrads, 1 G4 helix, 1 G4 stem · (2+2), UDUD
1 glyco-bond=s-s- groove=wnwn planarity=0.138 type=planar nts=4 GGGG A.DG1,A.DG9,B.DG8,B.DG2 2 glyco-bond=-s-s groove=wnwn planarity=0.141 type=planar nts=4 GGGG A.DG2,A.DG8,B.DG9,B.DG1