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PDB-id
1k4x
Class
DNA
Method
NMR
Summary
Potassium form of oxy-1.5 quadruplex DNA
Reference
Schultze, P., Hud, N.V., Smith, F.W., Feigon, J.: (1999) "The effect of sodium, potassium and ammonium ions on the conformation of the dimeric quadruplex formed by the Oxytricha nova telomere repeat oligonucleotide d(G(4)T(4)G(4))." Nucleic Acids Res., 27, 3018-3028.
Abstract
The DNA sequence d(G(4)T(4)G(4)) [Oxy-1.5] consists of 1.5 units of the repeat in telomeres of Oxytricha nova and has been shown by NMR and X-ray crystallographic analysis to form a dimeric quadruplex structure with four guanine-quartets. However, the structure reported in the X-ray study has a fundamentally different conformation and folding topology compared to the solution structure. In order to elucidate the possible role of different counterions in this discrepancy and to investigate the conformational effects and dynamics of ion binding to G-quadruplex DNA, we compare results from further experiments using a variety of counterions, namely K(+), Na(+)and NH(4)(+). A detailed structure determination of Oxy-1.5 in solution in the presence of K(+)shows the same folding topology as previously reported with the same molecule in the presence of Na(+). Both conformations are symmetric dimeric quadruplexes with T(4)loops which span the diagonal of the end quartets. The stack of quartets shows only small differences in the presence of K(+)versus Na(+)counterions, but the T(4)loops adopt notably distinguishable conformations. Dynamic NMR analysis of the spectra of Oxy-1.5 in mixed Na(+)/K(+)solution reveals that there are at least three K(+)binding sites. Additional experiments in the presence of NH(4)(+)reveal the same topology and loop conformation as in the K(+)form and allow the direct localization of three central ions in the stack of quartets and further show that there are no specific NH(4)(+)binding sites in the T(4)loop. The location of bound NH(4)(+)with respect to the expected coordination sites for Na(+)binding provides a rationale for the difference observed for the structure of the T(4)loop in the Na(+)form, with respect to that observed for the K(+)and NH(4)(+)forms.
G4 notes
2 G-tetrads, 1 G4 helix, 1 G4 stem · (2+2), UDDU

Base-block schematics in six views [summary · tetrads · helices · stems · costacks · homepage]

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List of 2 G-tetrads [summary · schematics · helices · stems · costacks · homepage]

 1 glyco-bond=-ss- groove=w-n- planarity=0.345 type=other  nts=4 GGGG A.DG2,B.DG3,A.DG11,B.DG10
 2 glyco-bond=s--s groove=w-n- planarity=0.345 type=other  nts=4 GGGG A.DG3,B.DG2,A.DG10,B.DG11

List of 1 G4-helix [summary · schematics · tetrads · stems · costacks · homepage]

In DSSR, a G4-helix is defined by stacking interactions of G-tetrads, regardless of backbone connectivity, and may contain more than one G4-stem.

Helix#1, 2 G-tetrad layers, inter-molecular, with 1 stem

 1  glyco-bond=-ss- groove=w-n- WC-->Major nts=4 GGGG A.DG2,B.DG3,A.DG11,B.DG10
 2  glyco-bond=s--s groove=w-n- Major-->WC nts=4 GGGG A.DG3,B.DG2,A.DG10,B.DG11
  step#1  pp(><,inward)   area=22.05 rise=3.18 twist=36.0
  strand#1 DNA glyco-bond=-s nts=2 GG A.DG2,A.DG3
  strand#2 DNA glyco-bond=s- nts=2 GG B.DG3,B.DG2
  strand#3 DNA glyco-bond=s- nts=2 GG A.DG11,A.DG10
  strand#4 DNA glyco-bond=-s nts=2 GG B.DG10,B.DG11

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1 stacking diagram
 1  glyco-bond=-ss- groove=w-n- WC-->Major nts=4 GGGG A.DG2,B.DG3,A.DG11,B.DG10
2 glyco-bond=s--s groove=w-n- Major-->WC nts=4 GGGG A.DG3,B.DG2,A.DG10,B.DG11
step#1 pp(><,inward) area=22.05 rise=3.18 twist=36.0

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List of 1 G4-stem [summary · schematics · tetrads · helices · costacks · homepage]

In DSSR, a G4-stem is defined as a G4-helix with backbone connectivity. Bulges are also allowed along each of the four strands.

Stem#1, 2 G-tetrad layers, 2 loops, inter-molecular, UDDU, anti-parallel, (2+2)

 1  glyco-bond=-ss- groove=w-n- WC-->Major nts=4 GGGG A.DG2,B.DG3,A.DG11,B.DG10
 2  glyco-bond=s--s groove=w-n- Major-->WC nts=4 GGGG A.DG3,B.DG2,A.DG10,B.DG11
  step#1  pp(><,inward)   area=22.05 rise=3.18 twist=36.0
  strand#1  U DNA glyco-bond=-s nts=2 GG A.DG2,A.DG3
  strand#2  D DNA glyco-bond=s- nts=2 GG B.DG3,B.DG2
  strand#3  D DNA glyco-bond=s- nts=2 GG A.DG11,A.DG10
  strand#4  U DNA glyco-bond=-s nts=2 GG B.DG10,B.DG11
  loop#1 type=diagonal  strands=[#1,#3] nts=6 GTTTTG A.DG4,A.DT5,A.DT6,A.DT7,A.DT8,A.DG9
  loop#2 type=diagonal  strands=[#2,#4] nts=6 GTTTTG B.DG4,B.DT5,B.DT6,B.DT7,B.DT8,B.DG9

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List of 0 G4 coaxial stacks [summary · schematics · tetrads · helices · stems · homepage]

List of 0 non-stem G4-loops (including the two closing Gs)