DSSR-derived G-quadruplex features in PDB entry 1y8d
Poster "DSSR-Enabled Automatic Identification and Annotation of G-quadruplexes in the PDB" presented at the RNA2020 online meeting
Citation: before a paper dedicated to the DSSR-G4 module comes out, please cite the 2015 DSSR paper published in Nucleic Acids Research.
- Dimeric parallel-stranded tetraplex with 3+1 5' G-tetrad interface, single-residue chain reversal loops and gag triad in the context of a(gggg) pentad
- Phan, A.T., Kuryavyi, V.V., Ma, J.-B., Faure, A., Andreola, M.-L., Patel, D.J.: (2005) "An interlocked dimeric parallel-stranded DNA quadruplex: A potent inhibitor of HIV-1 integrase." Proc.Natl.Acad.Sci.USA, 102, 634-639.
- We report on the NMR-based solution structure of the 93del d(GGGGTGGGAGGAGGGT) aptamer, a potent nanomolar inhibitor of HIV-1 integrase. This guanine-rich DNA sequence adopts an unusually stable dimeric quadruplex architecture in K+ solution. Within each 16-nt monomer subunit, which contains one A.(G.G.G.G) pentad sandwiched between two G.G.G.G tetrads, all G-stretches are parallel, and all guanines are anti with the exception of G1, which is syn. Dimer formation is achieved through mutual pairing of G1 of one monomer, with G2, G6, and G13 of the other monomer, to complete G.G.G.G tetrad formation. There are three single-nucleotide double-chain-reversal loops within each monomer fold, such that the first (T5) and third (A12) loops bridge three G-tetrad layers, whereas the second (A9) loop bridges two G-tetrad layers and participates in A.(G.G.G.G) pentad formation. Results of NMR and of integrase inhibition assays on loop-modified sequences allowed us to propose a strategy toward the potential design of improved HIV-1 integrase inhibitors. Finally, we propose a model, based on molecular docking approaches, for positioning the 93del dimeric DNA quadruplex within a basic channel/canyon formed between subunits of a dimer of dimers of HIV-1 integrase.
- G4 notes
- 6 G-tetrads, 1 G4 helix, 2 G4 stems, 1 G4 coaxial stack · 2(-P-P-P), parallel(4+0), UUUU · coaxial interfaces: 5'/5'-SEPARATED
1 glyco-bond=s--- groove=w--n planarity=0.091 type=planar nts=4 GGGG A.DG1,B.DG6,B.DG2,B.DG13 2 glyco-bond=--s- groove=-wn- planarity=0.091 type=planar nts=4 GGGG A.DG2,A.DG6,B.DG1,A.DG13 3 glyco-bond=---- groove=---- planarity=0.125 type=planar nts=4 GGGG A.DG3,A.DG7,A.DG10,A.DG14 4 glyco-bond=---- groove=---- planarity=0.141 type=planar nts=4 GGGG A.DG4,A.DG8,A.DG11,A.DG15 5 glyco-bond=---- groove=---- planarity=0.126 type=planar nts=4 GGGG B.DG3,B.DG7,B.DG10,B.DG14 6 glyco-bond=---- groove=---- planarity=0.141 type=planar nts=4 GGGG B.DG4,B.DG8,B.DG11,B.DG15
Helix#1, 6 G-tetrad layers, inter-molecular, with 2 stems
Stem#1, 2 G-tetrad layers, 3 loops, INTRA-molecular, UUUU, parallel, 2(-P-P-P), parallel(4+0)
Stem#2, 2 G-tetrad layers, 3 loops, INTRA-molecular, UUUU, parallel, 2(-P-P-P), parallel(4+0)
1 G4 helix#1 contains 2 G4 stems: [#1,#2] [5'/5'-SEPARATED]