DSSR-derived G-quadruplex features in PDB entry 2aqy
Poster "DSSR-Enabled Automatic Identification and Annotation of G-quadruplexes in the PDB" presented at the RNA2020 online meeting
Citation: before a paper dedicated to the DSSR-G4 module comes out, please cite the 2015 DSSR paper published in Nucleic Acids Research.
- (3+1) assembly of three human telomeric DNA repeats into an asymmetrical dimeric G-quadruplex
- Zhang, N., Phan, A.T., Patel, D.J.: (2005) "(3 + 1) Assembly of three human telomeric repeats into an asymmetric dimeric G-quadruplex." J.Am.Chem.Soc., 127, 17277-17285.
- We present an NMR study on the structure of a DNA fragment of the human telomere containing three guanine-tracts, d(GGGTTAGGGTTAGGGT). This sequence forms in Na(+) solution a unique asymmetric dimeric quadruplex, in which the G-tetrad core involves all three G-tracts of one strand and only the last 3'-end G-tract of the other strand. We show that a three-repeat human telomeric sequence can also associate with a single-repeat human telomeric sequence into a structure with the same topology that we name (3 + 1) quadruplex assembly. In this G-quadruplex assembly, there are one syn.syn.syn.anti and two anti.anti.anti.syn G-tetrads, two edgewise loops, three G-tracts oriented in one direction and the fourth oriented in the opposite direction. We discuss the possible implications of the new folding topology for understanding the structure of telomeric DNA, including t-loop formation, and for targeting G-quadruplexes in the telomeres.
- G4 notes
- 3 G-tetrads, 1 G4 helix, 1 G4 stem · (3+1), UUUD
1 glyco-bond=sss- groove=--wn planarity=0.171 type=other nts=4 GGGG A.DG1,B.DG19,A.DG13,A.DG9 2 glyco-bond=---s groove=--wn planarity=0.220 type=other nts=4 gGGG A.DI2,B.DG20,A.DG14,A.DG8 3 glyco-bond=---s groove=--wn planarity=0.383 type=bowl nts=4 GGGG A.DG3,B.DG21,A.DG15,A.DG7