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PDB-id
2kf8
Class
DNA
Method
NMR
Summary
Structure of a two-G-tetrad basket-type intramolecular G-quadruplex formed by human telomeric repeats in k+ solution
Reference
Lim, K.W., Amrane, S., Bouaziz, S., Xu, W., Mu, Y., Patel, D.J., Luu, K.N., Phan, A.T.: (2009) "Structure of the human telomere in K+ solution: a stable basket-type G-quadruplex with only two G-tetrad layers." J.Am.Chem.Soc., 131, 4301-4309.
Abstract
Previously, it has been reported that human telomeric DNA sequences could adopt in different experimental conditions four different intramolecular G-quadruplexes each involving three G-tetrad layers, namely, Na(+) solution antiparallel-stranded basket form, K(+) crystal parallel-stranded propeller form, K(+) solution (3 + 1) Form 1, and K(+) solution (3 + 1) Form 2. Here we present a new intramolecular G-quadruplex adopted by a four-repeat human telomeric sequence in K(+) solution (Form 3). This structure is a basket-type G-quadruplex with only two G-tetrad layers: loops are successively edgewise, diagonal, and edgewise; glycosidic conformations of guanines are syn x syn x anti x anti around each tetrad. Each strand of the core has both a parallel and an antiparallel adjacent strands; there are one narrow, one wide, and two medium grooves. Despite the presence of only two G-tetrads in the core, this structure is more stable than the three-G-tetrad intramolecular G-quadruplexes previously observed for human telomeric sequences in K(+) solution. Detailed structural elucidation of Form 3 revealed extensive base pairing and stacking in the loops capping both ends of the G-tetrad core, which might explain the high stability of the structure. This novel structure highlights the conformational heterogeneity of human telomeric DNA. It establishes a new folding principle for G-quadruplexes and suggests new loop sequences and structures for targeting in human telomeric DNA.
G4 notes
2 G-tetrads, 1 G4 helix, 1 G4 stem · 2(-LwD+Ln), basket(2+2), UDDU

Base-block schematics in six views [summary · tetrads · helices · stems · costacks · homepage]

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List of 2 G-tetrads [summary · schematics · helices · stems · costacks · homepage]

 1 glyco-bond=s--s groove=w-n- planarity=0.093 type=planar nts=4 GGGG A.DG1,A.DG8,A.DG20,A.DG14
 2 glyco-bond=-ss- groove=w-n- planarity=0.152 type=planar nts=4 GGGG A.DG2,A.DG7,A.DG19,A.DG15

List of 1 G4-helix [summary · schematics · tetrads · stems · costacks · homepage]

In DSSR, a G4-helix is defined by stacking interactions of G-tetrads, regardless of backbone connectivity, and may contain more than one G4-stem.

Helix#1, 2 G-tetrad layers, INTRA-molecular, with 1 stem

 1  glyco-bond=s--s groove=w-n- Major-->WC nts=4 GGGG A.DG1,A.DG8,A.DG20,A.DG14
 2  glyco-bond=-ss- groove=w-n- WC-->Major nts=4 GGGG A.DG2,A.DG7,A.DG19,A.DG15
  step#1  mm(<>,outward)  area=13.98 rise=3.21 twist=19.2
  strand#1 DNA glyco-bond=s- nts=2 GG A.DG1,A.DG2
  strand#2 DNA glyco-bond=-s nts=2 GG A.DG8,A.DG7
  strand#3 DNA glyco-bond=-s nts=2 GG A.DG20,A.DG19
  strand#4 DNA glyco-bond=s- nts=2 GG A.DG14,A.DG15

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1 stacking diagram
 1  glyco-bond=s--s groove=w-n- Major-->WC nts=4 GGGG A.DG1,A.DG8,A.DG20,A.DG14
2 glyco-bond=-ss- groove=w-n- WC-->Major nts=4 GGGG A.DG2,A.DG7,A.DG19,A.DG15
step#1 mm(<>,outward) area=13.98 rise=3.21 twist=19.2

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List of 1 G4-stem [summary · schematics · tetrads · helices · costacks · homepage]

In DSSR, a G4-stem is defined as a G4-helix with backbone connectivity. Bulges are also allowed along each of the four strands.

Stem#1, 2 G-tetrad layers, 3 loops, INTRA-molecular, UDDU, anti-parallel, 2(-LwD+Ln), basket(2+2)

 1  glyco-bond=s--s groove=w-n- Major-->WC nts=4 GGGG A.DG1,A.DG8,A.DG20,A.DG14
 2  glyco-bond=-ss- groove=w-n- WC-->Major nts=4 GGGG A.DG2,A.DG7,A.DG19,A.DG15
  step#1  mm(<>,outward)  area=13.98 rise=3.21 twist=19.2
  strand#1  U DNA glyco-bond=s- nts=2 GG A.DG1,A.DG2
  strand#2  D DNA glyco-bond=-s nts=2 GG A.DG8,A.DG7
  strand#3  D DNA glyco-bond=-s nts=2 GG A.DG20,A.DG19
  strand#4  U DNA glyco-bond=s- nts=2 GG A.DG14,A.DG15
  loop#1 type=lateral   strands=[#1,#2] nts=4 GTTA A.DG3,A.DT4,A.DT5,A.DA6
  loop#2 type=diagonal  strands=[#2,#4] nts=5 GTTAG A.DG9,A.DT10,A.DT11,A.DA12,A.DG13
  loop#3 type=lateral   strands=[#4,#3] nts=3 TTA A.DT16,A.DT17,A.DA18

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List of 0 G4 coaxial stacks [summary · schematics · tetrads · helices · stems · homepage]

List of 0 non-stem G4-loops (including the two closing Gs)