Summary information [schematics · tetrads · helices · stems · costacks · homepage]

PDB-id
6au4
Class
DNA
Method
X-ray (2.35 Å)
Summary
Crystal structure of the major quadruplex formed in the human c-myc promoter
Reference
Stump, S., Mou, T.C., Sprang, S.R., Natale, N.R., Beall, H.D.: (2018) "Crystal structure of the major quadruplex formed in the promoter region of the human c-MYC oncogene." PLoS ONE, 13, e0205584-e0205584.
Abstract
The c-MYC oncogene mediates multiple tumor cell survival pathways and is dysregulated or overexpressed in the majority of human cancers. The NHE III1 region of the c-MYC promoter forms a DNA quadruplex. Stabilization of this structure with small molecules has been shown to reduce expression of c-MYC, and targeting the c-MYC quadruplex has become an emerging strategy for development of antitumor compounds. Previous solution NMR studies of the c-MYC quadruplex have assigned the major conformer and topology of this important target, however, regions outside the G-quartet core were not as well-defined. Here, we report a high-resolution crystal structure (2.35 Å) of the major quadruplex formed in the NHE III1 region of the c-MYC promoter. The crystal structure is in general agreement with the solution NMR structure, however, key differences are observed in the position of nucleotides outside the G-quartet core. The crystal structure provides an alternative model that, along with comparisons to other reported quadruplex crystal structures, will be important to the rational design of selective compounds. This work will aid in development of ligands to target the c-MYC promoter quadruplex with the goal of creating novel anticancer therapies.
G4 notes
6 G-tetrads, 2 G4 helices, 2 G4 stems · 3(-P-P-P), parallel(4+0), UUUU

Base-block schematics in six views [summary · tetrads · helices · stems · costacks · homepage]

PyMOL session file PDB file View in 3Dmol.js

List of 6 G-tetrads [summary · schematics · helices · stems · costacks · homepage]

 1 glyco-bond=---- groove=---- planarity=0.043 type=planar nts=4 GGGG A.DG4,A.DG8,A.DG13,A.DG17
 2 glyco-bond=---- groove=---- planarity=0.130 type=planar nts=4 GGGG A.DG5,A.DG9,A.DG14,A.DG18
 3 glyco-bond=---- groove=---- planarity=0.197 type=bowl   nts=4 GGGG A.DG6,A.DG10,A.DG15,A.DG19
 4 glyco-bond=---- groove=---- planarity=0.074 type=planar nts=4 GGGG B.DG4,B.DG8,B.DG13,B.DG17
 5 glyco-bond=---- groove=---- planarity=0.140 type=planar nts=4 GGGG B.DG5,B.DG9,B.DG14,B.DG18
 6 glyco-bond=---- groove=---- planarity=0.249 type=bowl   nts=4 GGGG B.DG6,B.DG10,B.DG15,B.DG19

List of 2 G4-helices [summary · schematics · tetrads · stems · costacks · homepage]

In DSSR, a G4-helix is defined by stacking interactions of G-tetrads, regardless of backbone connectivity, and may contain more than one G4-stem.

Helix#1, 3 G-tetrad layers, INTRA-molecular, with 1 stem

 1  glyco-bond=---- groove=---- WC-->Major nts=4 GGGG A.DG4,A.DG8,A.DG13,A.DG17
 2  glyco-bond=---- groove=---- WC-->Major nts=4 GGGG A.DG5,A.DG9,A.DG14,A.DG18
 3  glyco-bond=---- groove=---- WC-->Major nts=4 GGGG A.DG6,A.DG10,A.DG15,A.DG19
  step#1  pm(>>,forward)  area=10.75 rise=3.36 twist=30.5
  step#2  pm(>>,forward)  area=10.96 rise=3.34 twist=29.0
  strand#1 DNA glyco-bond=--- nts=3 GGG A.DG4,A.DG5,A.DG6
  strand#2 DNA glyco-bond=--- nts=3 GGG A.DG8,A.DG9,A.DG10
  strand#3 DNA glyco-bond=--- nts=3 GGG A.DG13,A.DG14,A.DG15
  strand#4 DNA glyco-bond=--- nts=3 GGG A.DG17,A.DG18,A.DG19

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2 stacking diagrams
 1  glyco-bond=---- groove=---- WC-->Major nts=4 GGGG A.DG4,A.DG8,A.DG13,A.DG17
2 glyco-bond=---- groove=---- WC-->Major nts=4 GGGG A.DG5,A.DG9,A.DG14,A.DG18
step#1 pm(>>,forward) area=10.75 rise=3.36 twist=30.5

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 2  glyco-bond=---- groove=---- WC-->Major nts=4 GGGG A.DG5,A.DG9,A.DG14,A.DG18
3 glyco-bond=---- groove=---- WC-->Major nts=4 GGGG A.DG6,A.DG10,A.DG15,A.DG19
step#2 pm(>>,forward) area=10.96 rise=3.34 twist=29.0

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Helix#2, 3 G-tetrad layers, INTRA-molecular, with 1 stem

 1  glyco-bond=---- groove=---- WC-->Major nts=4 GGGG B.DG4,B.DG8,B.DG13,B.DG17
 2  glyco-bond=---- groove=---- WC-->Major nts=4 GGGG B.DG5,B.DG9,B.DG14,B.DG18
 3  glyco-bond=---- groove=---- WC-->Major nts=4 GGGG B.DG6,B.DG10,B.DG15,B.DG19
  step#1  pm(>>,forward)  area=10.11 rise=3.37 twist=30.8
  step#2  pm(>>,forward)  area=11.50 rise=3.47 twist=28.9
  strand#1 DNA glyco-bond=--- nts=3 GGG B.DG4,B.DG5,B.DG6
  strand#2 DNA glyco-bond=--- nts=3 GGG B.DG8,B.DG9,B.DG10
  strand#3 DNA glyco-bond=--- nts=3 GGG B.DG13,B.DG14,B.DG15
  strand#4 DNA glyco-bond=--- nts=3 GGG B.DG17,B.DG18,B.DG19

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2 stacking diagrams
 1  glyco-bond=---- groove=---- WC-->Major nts=4 GGGG B.DG4,B.DG8,B.DG13,B.DG17
2 glyco-bond=---- groove=---- WC-->Major nts=4 GGGG B.DG5,B.DG9,B.DG14,B.DG18
step#1 pm(>>,forward) area=10.11 rise=3.37 twist=30.8

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 2  glyco-bond=---- groove=---- WC-->Major nts=4 GGGG B.DG5,B.DG9,B.DG14,B.DG18
3 glyco-bond=---- groove=---- WC-->Major nts=4 GGGG B.DG6,B.DG10,B.DG15,B.DG19
step#2 pm(>>,forward) area=11.50 rise=3.47 twist=28.9

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List of 2 G4-stems [summary · schematics · tetrads · helices · costacks · homepage]

In DSSR, a G4-stem is defined as a G4-helix with backbone connectivity. Bulges are also allowed along each of the four strands.

Stem#1, 3 G-tetrad layers, 3 loops, INTRA-molecular, UUUU, parallel, 3(-P-P-P), parallel(4+0)

 1  glyco-bond=---- groove=---- WC-->Major nts=4 GGGG A.DG4,A.DG8,A.DG13,A.DG17
 2  glyco-bond=---- groove=---- WC-->Major nts=4 GGGG A.DG5,A.DG9,A.DG14,A.DG18
 3  glyco-bond=---- groove=---- WC-->Major nts=4 GGGG A.DG6,A.DG10,A.DG15,A.DG19
  step#1  pm(>>,forward)  area=10.75 rise=3.36 twist=30.5
  step#2  pm(>>,forward)  area=10.96 rise=3.34 twist=29.0
  strand#1  U DNA glyco-bond=--- nts=3 GGG A.DG4,A.DG5,A.DG6
  strand#2  U DNA glyco-bond=--- nts=3 GGG A.DG8,A.DG9,A.DG10
  strand#3  U DNA glyco-bond=--- nts=3 GGG A.DG13,A.DG14,A.DG15
  strand#4  U DNA glyco-bond=--- nts=3 GGG A.DG17,A.DG18,A.DG19
  loop#1 type=propeller strands=[#1,#2] nts=1 T A.DT7
  loop#2 type=propeller strands=[#2,#3] nts=2 TA A.DT11,A.DA12
  loop#3 type=propeller strands=[#3,#4] nts=1 T A.DT16

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Stem#2, 3 G-tetrad layers, 3 loops, INTRA-molecular, UUUU, parallel, 3(-P-P-P), parallel(4+0)

 1  glyco-bond=---- groove=---- WC-->Major nts=4 GGGG B.DG4,B.DG8,B.DG13,B.DG17
 2  glyco-bond=---- groove=---- WC-->Major nts=4 GGGG B.DG5,B.DG9,B.DG14,B.DG18
 3  glyco-bond=---- groove=---- WC-->Major nts=4 GGGG B.DG6,B.DG10,B.DG15,B.DG19
  step#1  pm(>>,forward)  area=10.11 rise=3.37 twist=30.8
  step#2  pm(>>,forward)  area=11.50 rise=3.47 twist=28.9
  strand#1  U DNA glyco-bond=--- nts=3 GGG B.DG4,B.DG5,B.DG6
  strand#2  U DNA glyco-bond=--- nts=3 GGG B.DG8,B.DG9,B.DG10
  strand#3  U DNA glyco-bond=--- nts=3 GGG B.DG13,B.DG14,B.DG15
  strand#4  U DNA glyco-bond=--- nts=3 GGG B.DG17,B.DG18,B.DG19
  loop#1 type=propeller strands=[#1,#2] nts=1 T B.DT7
  loop#2 type=propeller strands=[#2,#3] nts=2 TA B.DT11,B.DA12
  loop#3 type=propeller strands=[#3,#4] nts=1 T B.DT16

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List of 0 G4 coaxial stacks [summary · schematics · tetrads · helices · stems · homepage]

List of 0 non-stem G4-loops (including the two closing Gs)